Journal: The Journal of Neuroscience

Article Title: Trans-Synaptic Stimulation of Cortical Acetylcholine Release after Partial 192 IgG-Saporin-Induced Loss of Cortical Cholinergic Afferents

doi: 10.1523/JNEUROSCI.16-20-06592.1996

Figure Lengend Snippet: Representative coronal brain sections (stained for AChE) from a sham-lesioned animal (A) and a 192 IgG-saporin-lesioned animal (B) demonstrating representative placement of the microdialysis probe in the frontoparietal cortex. Again, the lesioned cortex shows a marked reduction in AChE-positive fiber staining. C andD show coronal sections through the basal forebrain area of representative control and lesioned animals, respectively. AChE-positive fiber staining is markedly reduced in the lesioned basal forebrain, implying a loss of cholinergic neurons in the basal forebrain after cortical infusion of 192 IgG-saporin. Eand F schematically illustrate the anatomical zones present in C and D. GP, Globus pallidus; SI, substantia innominata;CPu, caudate putamen. Magnification: A,B, 5×; C, D, 25×.

Article Snippet: Two weeks after the initial surgery, each animal was implanted with a stainless steel microdialysis guide cannula (0.65 mm outer diameter; CMA Microdialysis, Acton, MA) at a 45° angle into the right frontoparietal cortex through the hole previously drilled for the middle toxin or vehicle infusion site.

Techniques: Staining, Control

Journal: The Journal of Neuroscience

Article Title: Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression

doi: 10.1523/JNEUROSCI.3717-16.2017

Figure Lengend Snippet: Surface expression of GluA1 and GluA2 and total protein expression of xCT and GLT-1. Cocaine-naive rats received intra-NAc AS directed at GLT-1 (n = 5) or Ctrl oligo (n = 5). Protein expression was expressed as a percentage of the Ctrl group. A, GLT-1 antisense decreased total protein expression of GLT-1. B, The infusion of GLT-1 AS had no effect on total xCT expression. C, Surface GluA1 was not affected by GLT-1 knockdown. D, Surface GluA2 was not affected by GLT-1 knockdown. *p < 0.05 compared to Ctrl. C, Ctrl oligo; G, GLT-1 AS.

Article Snippet: For the implantation of guide cannulae, rats were placed in a stereotaxic frame (Stoelting), and stainless steel guide cannulae for antisense infusion (26 gauge; Plastics One) or microdialysis (22 gauge; Synaptech) were aimed at the NAc according to the following coordinates (anteroposterior, +1.2 mm; mediolateral, ±1.6 mm; dorsoventral, −5.5 mm; Paxinos and Watson, 2005 ).

Techniques: Expressing

Journal: The Journal of Neuroscience

Article Title: Contrasting the Role of xCT and GLT-1 Upregulation in the Ability of Ceftriaxone to Attenuate the Cue-Induced Reinstatement of Cocaine Seeking and Normalize AMPA Receptor Subunit Expression

doi: 10.1523/JNEUROSCI.3717-16.2017

Figure Lengend Snippet: No-net-flux microdialysis revealed that basal extracellular glutamate is reduced by intra-NAc infusion of xCT antisense. A, The concentration of glutamate perfused was plotted against the net flux of glutamate. B, Computing the point of no net-flux across the dialysis membrane in A and making a comparison between groups revealed that xCT antisense significantly reduced the basal glutamate level relative to the Ctrl group. C, Microdialysis probe tracks through the NAc; representative examples of dialysis probes are depicted along the rostrocaudal axis of the NAc. *Indicates significant difference between groups (p < 0.05).

Article Snippet: For the implantation of guide cannulae, rats were placed in a stereotaxic frame (Stoelting), and stainless steel guide cannulae for antisense infusion (26 gauge; Plastics One) or microdialysis (22 gauge; Synaptech) were aimed at the NAc according to the following coordinates (anteroposterior, +1.2 mm; mediolateral, ±1.6 mm; dorsoventral, −5.5 mm; Paxinos and Watson, 2005 ).

Techniques: Concentration Assay